Part:BBa_K562004
Salty_PduJK BMC Components
This is a composite part comprising a constituive promoter (identical to that from the basic part BBa_K562000), which is the tatABCD promoter from E. coli K-12, driving production of the PduJ and PduK proteins from Salmonella enterica serovar Typhimurium LT2. These are components of the Salmonella bacterial microcompartment (BMC). The PduK gene product carries a C-terminal 6-His affinity/epitope tag. Production of the PduK protein has been verified by Western immunoblotting (anti-penta-His) and production of both PduJ and PduK have been confirmed by 35S-Met labelling. The proteins have also been purified from E. coli by immobilised metal affinity chromatography (IMAC) and identified by tryptic peptide mass fingerprinting. The construct is cloned as an EcoRI / PstI fragment into pSB1C3. The clone is also known as pSB-JK in the Sargent Laboratory, Dundee, UK.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 666
Illegal BamHI site found at 805
Illegal BamHI site found at 899 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 293
Illegal AgeI site found at 560
Illegal AgeI site found at 596 - 1000COMPATIBLE WITH RFC[1000]
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